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  • Subject area(s): Science
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  • Published on: 15th October 2019
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The original article, found in the ‘Science Daily, online magazine, focused on the development of a new microscope technique. This technique allows researchers to have a more in-depth view of the internal structure of the embryo, which they believe will be beneficial in the area of fertility treatments.

The ‘Science Daily’ report refers back to an article on ‘Gradient Light Interference Microscopy (GLIM) for 3D imaging of unlabeled specimens. GLIM was introduced as a new method of Quantitative Phase Imaging (QPI), able to give exact value phase for thin samples. It can also be used for tomography allowing researchers to obtain three-dimensional information from thick samples. This new microscope was used on a variety of samples ranging from beads to bovine embryos and can be used to image living cells nondestructively. (Nguyēn, Kandel, Rubessa, Wheeler & Popescu, 2017)

This research was completed by Tan H. Nguyen and Mikhail E. Kandel under the supervision of Professor Gabriel Popescu and Matthew Wheeler. And  Tan H. Nguyen was a 2015 fellow in the Computational Science and Engineering program with a focus on Electrical and Computer Engineering at the University of Illinois Urbana- Champaign where he all earned his Bachelor of Engineering in Automation. Nguyen worked with the department of electrical and computer engineering to develop new computational tools for better diagnosis of prostate cancer. Mikhail Eugene Kandel is a graduate student and graduate advisor to Professor Popescu. Whose primary area of research are Biomedical Imaging, Bioengineering and Acoustics, biophotonics and biomedical optics. He currently teaches Optical Imaging and Digital Systems Laboratory at the University of Illinois. Gabriel Popescu is an Associate professor in Electrical and Computer Engineering at the University of Illinois at Urbana-Champaign. He possesses both a Bachelors and Masters in Physics from the University of Bucharest as well as a Masters and PhD in Optics from the University of Florida. Currently, he is an Associate Professor at Illinois where he directs the quantitative light imaging laboratory at the Beckman Institute for Advanced Science and Technology.

Their initial research was later cited in a paper entitled “High-throughput diffraction tomography with a computational microscope”, in reference to the tomography methodologies developed over time. In the article, the researchers demonstrated a scan free three-dimensional phase and absorption reconstruction using intensity only measurements (Ling, Tahir, Lin, Lee& Tian, 2018).  Researchers used both a sparse and thick cell sample to test the technique and found that this technique allowed for data acquisition and computation as well as memory efficiency. This system is convenient as it can be built onto a standard commercial microscope with a LED array source add-on and increases the application reach with minimum hardware adjustments (Ling, Tahir, Lin, Lee& Tian, 2018).

The contributing authors of this research were Ruilong Ling, Waleed Tahir, Hsing-Ying Lin, Hakho Lee and Lei Tan.  They worked collaboratively with the assistance of funding from the National Science Foundation Industry and University Cooperative Research centre of Biophotonic Sensors and Systems. Waleed Tahir is a PhD student in the Electrical and Computer Engineering who possesses a bachelor in Avionics Engineering from the University of Science and Technology in Pakistan. Ruilong Ling is a Masters student. Lei Tan is the Assistant Professor of Electrical and computer engineering at the University of Boston. He earned his PhD and masters at the Massachusetts Institute of Technology and his Bachelors at Tsinghua University receiving various scholarships and recognition throughout his academic career. Hakho Lee is an associate professor of Radiology at Harvard Medical School and the director of the Biomedical Engineering Program and the Centre for Systems Biology at the Massachusetts General Hospital. He received his PhD in Physics from Harvard University and possesses extensive experience in the areas of nanomaterials, biophysics, microfluidics and electrical engineering. Hsing-Ying Lin is a Postdoctoral Fellow under the tutelage of Hakho Lee at the Centre for Systems Biology at the Massachusetts General Hospital

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