Bifunctional activity of the ent-kaurene oxidase from Montanoa tomentosa and its N-terminal modified version for production of isokaurenoic acid and kaurenoic acid in engineered yeasts
We report the bifunctional activity of the native ent-kaurene oxidase from Montanoa tomentosa (MtKO) and its N-terminal modified version (LMtKO) for producing both isokaurenoic acid and kaurenoic acid in Saccharomyces cerevisiae. The Km app of MtKO showed more affinity for ent-kaurene (80.5 µM) than for isokaurene (96.4 µM). Interestingly, LMtKO exhibited an increase of … Read more