Successful Creation of Recombinant pET-41a(+)/EGFP Plasmid Confirmed by PCR and Restriction Digest
Introduction Plasmids are so helpful and important to understanding about genes in the field genetic. Fragment of DNA can ligate to plasmid vector to create a recombinant expression plasmid. In 1973, recombinant plasmid DNA was first achieved by Boyer and Cohen at Stanford University, California. In the beginning of this experiment, we will create a … Read more