Essay: Antimicrobial activity of different toothpastes on dental bacteria

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This in-vitro experiment conducted to test antimicrobial activity of different toothpastes on dental bacteria. Five brands of toothpastes namely Colgate Total, Oral-B Pro-expert, Siwak.F, Aloe Dent and Dabur Herbal were tested for their antimicrobial activity on Staphylococcus auresus, Staphylococcus epidermidis, E-coli and cariogenic bacteria Streptococcus mutans using agar disc diffusion method at different concentrations, MIC & MBC tests. The study proved that fluoride-containing toothpaste with triclosan was most potent than fluoride-containing toothpaste without triclosan, and herbal toothpaste with and without fluoride. The variations in antimicrobial activity of tested toothpastes were run simultaneously against broad-spectrum antibiotic ampicillin and chloramphenicol. The activity of these toothpastes is directly related to presence of antimicrobial compositions in their formulations. As a result, these brands of toothpastes can be used to control the oral biofilms and periodontal diseases related to the tested organism.
This independent study was conducted in King Saud University, Riyadh, Saudi Arabia under supervision of Mrs. Basmah Almaarik, faculty member at Clinical Laboratory Sciences department of Applied Medical Sciences College in King Saud University.
Laboratory work was performed by me personally, my supervisor and my colleagues Hebah Sharifi, Fedhah Alshalawi, Jawaher Ebrahim and Saida Omar.
I’d like to express my sincere gratitude to my supervisor, Mrs. Basmah Almaarik, who enlightened me with her experience and knowledge, and for always being supportive and kind at all times.
I’d also like to point out how cooperative and kind the whole university staff members and students were. They were always more than willing to offer help with great dedication.
Bacterial organism Streptococcus mutans was a courtesy of Dr. Sara Algahtani, faculty member at Dentistry College in King Saud University and Mrs. Gehan Al-sharif, laboratory technician in King Saud University.
Abbreviations Meanings
< Less than
> More than
˚C Degrees Celsius
A. baumannii Acinetobacter baumannii
CDC Centers for Disease Control and Prevention
ATCC American Type Culture Collection
Co Q10 Ubiquinone
CO2 Carbon dioxide
E. coli Escherichia coli
E. faecalis Enterococcus faecalis
ECHA European Chemicals Agency
EPA Environmental Protection Agency of United States
EU European Union
FDA Food and Drug Administration of United States
g/ml Gram per millilitre
K. pneumonia Klebsiella pneumonia
KSU King Saud University
MBC Minimum bactericidal concentration
MDR Multi-drug resistant
mg/ml Milligram per millilitre
MIC Minimum inhibitory concentration
ml Millilitre
mm millimeters
MRSA Methicillin-resistant Staphylococcus aureus
MRSE Methicillin-resistant Staphylococcus epidermidis
P. aeruginosa Pseudomonas aeruginosa
pH Hydrogen ion concentration
ppm Parts per million
S. maltophilia Stenotrophomonas maltophilia
S. marcescens Serratia marcescens
S. mutants Streptococcus mutans
SPF Sun protecting factor
Staph. aureus Staphylococcus aureus
Staph. epidermidis Staphylococus epidermidis
US United States of America
UK United Kingdom
vs. Versus
w/w Weight of solute by weight of total solution
WHO World Health Organization
Biofilms 1
Streptococcus mutans 2
Vaccination against Streptococcus mutans 3
Staphylococcus aureus, Staphylococcus epidermidis & E-coli 4
Toothpaste: Herbal vs. Regular 5
Tricloasn vs Fluoride 5
Herbal Toothpastes 7
Active Natural Ingredients in the Selected …

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