Gene editing methods (HF-BE3, HF-Cas9)

Most genome-editing methods rely on delivery of exogenous plasmid or viral DNA into cells to express the necessary editing tools intracellularly. However, since the expression is uncontrollable once the plasmid is expressed, sometimes the produced nucleases like Cas9 and sgRNA will lead to the excessive off-target editing after the on-target editing. To solve this problem, … Read more

CRISPR/Cas9 in medicine, antibiotic resistance, agriculture & ethical considerations

Genetic engineering has become readily approachable since the development of the latest gene editing tool CRISPR/Cas9 in 2012. This pioneering technology holds the promise of modifying genomes of any organism in the world, curing genetic diseases and reshaping the biosphere to benefit the environment (Baltimore et al, 2015). However, there are number of ethical and … Read more

Human manipulation techniques relating to transgenesis & selective breeding of cows

Introduction The following biological report discusses the human manipulation techniques relating to transgenesis and selective breeding of cows. Transgenesis refers to the removal of beta-lactoglobulin protein from milk produced by cows to reduce immune reactions due to allergies to the protein. This is done through multiple techniques; this report refers to CRISPR-Cas9 (Clustered Regularly Interspaced … Read more

Applications for CRISPR-Cas (Glaucoma, Cancer, Cystic Fibrosis, Crops)

Introduction In 1987, scientists came across the concept of CRISPR when they noticed an unexpected chain of DNA sequences that were altered and modified, which was then recognised as a CRISPR in Escherichia coli in the course of gene analysis. Once CRISPR was discovered all bacterial species were distinguished by short sequences similar to other … Read more

Biological implications of using the CRISPR/Cas9 gene drive

Predator Free 2050 (PF2050) is a New Zealand Government enforced plan with the goal to eradicating New Zealand’s introduced pests by 2050. The newest proposal is genetic engineering and modification of Possum’s DNA, through a process of CRISPR/Cas9-gene-drives to slowly eliminate those which harm NZ’s endemic species. CRISPR stands for Clustered Regularly Interspaced Short Palindromic … Read more

Biotechnology ethical issues (essay plan)

What is biotechnology and what are some of the ethical issues that arise from the biotechnological techniques? Biotechnology – The implementation of technology to maximise the use of biological organisms for human use. Benefits of Biotechnology: Medical Biotechnology: – Pharmaceuticals: Vaccines: Understanding and being able to strengthen the humans immune system Antibiotics: Synthetically designed drugs … Read more

Articles review – gene editing in neurodegenerative diseases

Scientific Literature Essay The purpose of this essay is to analyse the contents of a secondary (‘The Role of Gene Editing in Neurodegenerative Diseases’) and primary article (‘Modeling familial Alzheimer’s disease with induced pluripotent stem cells’) and then compare the two. The journal the review article is associated to must also be examined for a … Read more

The future of the CRISPR Cas9 genome editing system

Genome editing is a kind of genetic engineering in which scientists are able to change an organism’s DNA. This technology allows specific genes to be inserted, replaced, modified, and deleted. One type of gene editing is known as the CRISPR-Cas9 system (or clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9). The CRISPR-Cas9 system … Read more

Should gene editing be permitted?

Designer bags, designer belts, designer shoes, designer clothing. But, what about designer babies? Imagine if you already knew your unborn child would have Beyoncé’s voice, Usain bolt’s speed, the intelligence of Albert Einstein, Angelina Jolie’s cheekbones and Channing Tatum’s abs? Seems pretty farfetched but is it really? A designer baby is a human embryo that … Read more

Gene editing – should we?

The human race is approaching a new frontier in medical science: Genetic engineering. With the new CRISPR technology we have, we are able to edit genes much more precisely than we used to be, and we will be able to alter the human genome. Essentially, Gene editing is changing the body’s genes by altering or removing … Read more

Gene-editing and other uses of CRISPR

One of the most significant recent scientific discoveries was that of CRISPR, a bacterial immune system mechanism. CRISPR stands for “clustered regularly interspaced short palindromic repeats.” It may have a complex name, but the mechanism itself only involves 2 components: an enzyme that cuts DNA and a “guide” RNA strand. The CRISPR sequences themselves are … Read more

Anti-CRISPR mechanisms could enhance the genome editing process

Abstract Bacteria and bacteriophages have been involved in an evolutionary arms race for billions of years. The basis of the arms race results from bacteria evolving defense mechanisms to protect and prevent themselves from phage infection, while phages coevolve to evade these defense mechanisms. One of the mechanisms of defense bacteria use is the CRISPR-Cas … Read more