Expression & Purification of recombinant Green Fluorescent Protein (rGFP) from E. coli
Abstract: Green fluorescent protein (GFP) is a bioluminescent protein that is found in Aequorea Victoria, a species of Jelly fish. The goal of the experiment was to express and purify a recombinant form of GFP (rGFP) using Ni2+ agarose affinity chromatography technology. The His6/Xpress epitope tagged rGFP gene’s overexpression occurred in the BL21 (DE3) <pLysS> … Read more